[Histonet] Sakura film coverslipper xylene residue issue

[Jason McGough]

It has nothing to do with your coverslipper. Ours is from the mid 90's  
and we have had the brown spots a couple of times. We found that there  
to be water in our xylene. The little water droplets on the side of a  
container will cause this. Make sure everything that holds xylene is  
completely dry. Once we did this, we had no more brown spots. Good luck!

As far as xylene residue, it is your dispensing volume on the  
coverslipper that is causing this. Try turning the flow down and see  
what happens.

Jason McGough HT(ASCP)
Account Representative-Anatomic Pathology
Clinical Laboratory of the Black Hills
2805 5th Street Suite 210
Rapid City, SD 57701
605-343-2267
jmcgough <@t> clinlab.com


Quoting Jackie M O'Connor <Jackie.O'Connor <@t> abbott.com>:

> I have an older Sakura coverslipper, circa 1997ish.  We have noticed an
> increasing number of brown spots on tissue sections recently.   This does
> not occur if the same slides are covered with glass coverslips.   It does
> not look like a drying artefact, occurs randomly throughout tissue types,
> location on tissue, i.e., edge, center.   I've even showed these spots to
> the rep who admitted she has never seen this artefact before.   We use
> only the Tissue Tek tape, we maintain the equipment on a regular basis.
> The xylene flow is good.   Our only problem are these random brown spots.
>  Any suggestions?
> Jackie O'
>
>
>
> "Karin Groeger" <KGroeger <@t> USLABS.net>
> Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
> 05/29/2009 08:19 AM
>
> To
> "Scott" <lscott <@t> sfcn.org>, <Histonet <@t> lists.utsouthwestern.edu>
> cc
>
> Subject
> RE: [Histonet] Sakura film coverslipper xylene residue issue
>
>
>
>
>
>
> Hi Scott,
>   We place ours in front of a fan to dry if we are in a hurry, even if
> we air dry them we do not have a problem with the xylene residue,
> perhaps you have  a bad batch, check your lot # and call Sakura.  We
> coverslip over 1,000 slides a day and have not seen this.
>
> Karin Groeger
>
> Histology Supervisor
>
> US LABS, Irvine,CA
>
> 949-450-0145 ext. 649
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Scott
> Sent: Thursday, May 28, 2009 10:03 PM
> To: Histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Sakura film coverslipper xylene residue issue
>
> Hi,
>     We have a newer Sakura film coverslipper, does anyone have
> experience with this piece of equipment. I have a problem with the
> slides having a light xylene residue, after the slides come off of the
> coverslipper.
>
> How do you dry them? If I leave the slides on the coverslipper to air
> dry the xylene residue is noticeable. If I lightly wipe off all of the
> slides as they come off the coverslipper they look clean.
>
> How do you guys do it? I think it is a waste of time to wipe off all of
> the slides. I don't think the xylene flow is too high.
>
> Thanks for your help,
>
> Scott Hendricksen HT(ASCP)
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