[Histonet] Sakura film coverslipper xylene residue issue

[Jackie M O'Connor]

I have an older Sakura coverslipper, circa 1997ish.  We have noticed an 
increasing number of brown spots on tissue sections recently.   This does 
not occur if the same slides are covered with glass coverslips.   It does 
not look like a drying artefact, occurs randomly throughout tissue types, 
location on tissue, i.e., edge, center.   I've even showed these spots to 
the rep who admitted she has never seen this artefact before.   We use 
only the Tissue Tek tape, we maintain the equipment on a regular basis. 
The xylene flow is good.   Our only problem are these random brown spots.  
 Any suggestions?
Jackie O'



"Karin Groeger" <KGroeger <@t> USLABS.net> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
05/29/2009 08:19 AM

To
"Scott" <lscott <@t> sfcn.org>, <Histonet <@t> lists.utsouthwestern.edu>
cc

Subject
RE: [Histonet] Sakura film coverslipper xylene residue issue






Hi Scott,
  We place ours in front of a fan to dry if we are in a hurry, even if
we air dry them we do not have a problem with the xylene residue,
perhaps you have  a bad batch, check your lot # and call Sakura.  We
coverslip over 1,000 slides a day and have not seen this.

Karin Groeger

Histology Supervisor

US LABS, Irvine,CA

949-450-0145 ext. 649

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Scott
Sent: Thursday, May 28, 2009 10:03 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Sakura film coverslipper xylene residue issue

Hi,
    We have a newer Sakura film coverslipper, does anyone have
experience with this piece of equipment. I have a problem with the
slides having a light xylene residue, after the slides come off of the
coverslipper. 

How do you dry them? If I leave the slides on the coverslipper to air
dry the xylene residue is noticeable. If I lightly wipe off all of the
slides as they come off the coverslipper they look clean.

How do you guys do it? I think it is a waste of time to wipe off all of
the slides. I don't think the xylene flow is too high.

Thanks for your help,

Scott Hendricksen HT(ASCP)
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