[Histonet] Sakura film coverslipper xylene residue issue

[Mike Pence]

I have used this coverslipper for over 10 years and what I have found is
to allow the slides to dry under a fume hood in the rack as they come
off the coverslipper for 5 minutes. This allows the excess xylene to run
to the frosted end of the slide.

Also, the correct amount for dispensing xylene on the slide is a "string
of pearls" drop placement. This leaves about 5mm between drops from
beginning to end of slide.

Mike

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Scott
Sent: Friday, May 29, 2009 12:03 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Sakura film coverslipper xylene residue issue


Hi,
    We have a newer Sakura film coverslipper, does anyone have
experience with this piece of equipment. I have a problem with the
slides having a light xylene residue, after the slides come off of the
coverslipper. 

How do you dry them? If I leave the slides on the coverslipper to air
dry the xylene residue is noticeable. If I lightly wipe off all of the
slides as they come off the coverslipper they look clean.

How do you guys do it? I think it is a waste of time to wipe off all of
the slides. I don't think the xylene flow is too high.

Thanks for your help,

Scott Hendricksen HT(ASCP)
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