[Histonet] RE: CD8 or CD4 non Mouse primary

[C.M. van der Loos]

Hi Mark,Mouse CD4, clone 4B12 and Rabbit monoclonal CD8, clone SP16 is a good choice for your triple. Lots of success!Chris Chris van der Loos, PhD
Dept. of Pathology
Academic Medical Center M2-230
Meibergdreef 9
NL-1105 AZ Amsterdam
The Netherlands Date: Fri, 22 May 2009 10:22:14 -0700
From: "Mark Elliott" <MElliott <@t> mrl.ubc.ca>
Subject: [Histonet] CD8 or CD4 non Mouse primary
To: histonet <@t> lists.utsouthwestern.edu

Hi everyone.

We are needing to do a triple stain for IF using MHC Class II and CD4 and CD8.  I have Mouse monoclonals to CD4 and CD8 and we have a goat anti-MHC.  We were wondering if anyone knows of a good CD4 or CD 8 that is made in another species that we could use to avoid using two mouse antibodies. We are doing this on human lung.

Thanks

Mark