[Histonet] Whole rat skull decalcification protocol
TF
tifei <@t> foxmail.com
Mon May 25 02:53:20 CDT 2009
Hi alle
i am writing to see if you can provide me any useful protocol in removing the calcium from whole rat skull fixed in PFA for frozen sections later on.
I noticed there is use of 12% formic acid mixed in 6.25% formalin;
or 4% HCl mixed with 8% formic acid;
or, in a gentle but slower way, 14% tetrasodium EDTA (pH 7.4-7.6)
Anyone has other good sugestions to do so?
Thanks!
2009-05-25
TF
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