[Histonet] Whole rat skull decalcification protocol

[TF]

Hi alle
i am writing to see if you can provide me any useful protocol in removing the calcium from whole rat skull fixed in PFA for frozen sections later on.

I noticed there is use of 12% formic acid mixed in 6.25% formalin;
                              or 4% HCl mixed with 8% formic acid;
or, in a gentle but slower way, 14% tetrasodium EDTA (pH 7.4-7.6)

Anyone has other good sugestions to do so?
Thanks!

2009-05-25 



TF