[Histonet] IHC question- freezing aliquots

tdobersztyn <@t> chmca.org tdobersztyn <@t> chmca.org
Thu May 21 13:19:22 CDT 2009


Hello all! 

I have an antibody that has a "2" month expiration date.
It is recommended that we aliquot, and freeze (-80) the antibody- per the 
vendor.


How do you interpret: 
The CAP reg pertaining to expired antibody use: ANP .22432     ???????

"Are all immunohistochemical reagents used within their indicated dates? 

How are other institutions managing their antibodies that require 
freezing.--- do you aliquot,freeze, and give the reagent an extended 
expiration after it has been diluted? 


Thanks in advance!!!

I have read the past posts on this in the archives, but am wondering if 
there is a clear answer to antibody use and freezing. 


Theresa R Dobersztyn  HT (ASCP)
Electron Microscopy/Histology Labs
Senior Technologist
Department of Pathology
Akron Children's Hospital
1 Perkins Square
Akron, Ohio 44308

330-543-8279

Akron Children's Hospital - Proud winner of the NorthCoast 99 "Best
Workplace" award! 

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