[Histonet] IHC question- freezing aliquots

[Sebree Linda A]

In a similar situation we have gotten documentation (e-mail) from the
vendor stating the recommendation to freeze aliquots and to what degree
that extends the shelf life.  We haven't been queried on these
particular antibodies by an inspector but our supervisor feels that this
satisfies the CAP reg.

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
tdobersztyn <@t> chmca.org
Sent: Thursday, May 21, 2009 1:19 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] IHC question- freezing aliquots


Hello all! 

I have an antibody that has a "2" month expiration date.
It is recommended that we aliquot, and freeze (-80) the antibody- per
the 
vendor.


How do you interpret: 
The CAP reg pertaining to expired antibody use: ANP .22432     ???????

"Are all immunohistochemical reagents used within their indicated dates?


How are other institutions managing their antibodies that require 
freezing.--- do you aliquot,freeze, and give the reagent an extended 
expiration after it has been diluted? 


Thanks in advance!!!

I have read the past posts on this in the archives, but am wondering if 
there is a clear answer to antibody use and freezing. 


Theresa R Dobersztyn  HT (ASCP)
Electron Microscopy/Histology Labs
Senior Technologist
Department of Pathology
Akron Children's Hospital
1 Perkins Square
Akron, Ohio 44308

330-543-8279

Akron Children's Hospital - Proud winner of the NorthCoast 99 "Best
Workplace" award! 

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