[Histonet] bone tissue

[Jack Ratliff]

Haupts Gelatin works really well at a 1:1 ratio (Concentrate:50% EtOH). The only limitation is background staining from say a hematoxylin counterstain after the IHC. You can purchase the concentrate from Fisher (Haupts Fixative #785-71).

 

Jack


 
> Date: Thu, 21 May 2009 10:12:23 -0500
> From: Reuel.Cornelia <@t> tsrh.org
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] bone tissue
> 
> Can somebody help me with my bone tissue to remain intact during antigen retrieval for IHC staining. I have used subbed slides but It did not help me. Is there more better adhesive. Does Haupts Gelatin works better? I know this is a hundred year old question and hopefully this problem have been resolved since my undated knowledge could remember. 
> I would like to thank everybody for responding to my last e-mail on gross photography. It gives me more idea what to purchase. Thanks again histonetters and I really appreciate your help.
> 
> 
> 
> Reuel Cornelia, BS MT, AMT
> Cellular Pathology
> Texas Scottish Rite Hospital for Children
> 2222 Welborn Street
> Dallas, TX 75219
> Tel: 214-559-7766
> fax: 214-559-7768
> 
> 
> 
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