[Histonet] Embeeding frozen tissue in paraffin

[Patsy Ruegg]

No you need to thaw/fix in formalin or alcoholic fixative in order to
process and embed in paraffin.
Patsy


Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
email pruegg <@t> ihctech.net
website www.ihctech.net
IHC Resource Group www.ihcrg.org 


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Monfils,
Paul
Sent: Friday, May 15, 2009 10:41 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Embeeding frozen tissue in paraffin

I assume you mean the tissue was frozen without fixation, not that you want
to put it into paraffin without fixation?  No tissue will withstand paraffin
processing without fixation. The best way to turn a frozen tissue into a
paraffin embedded tissue is to drop the frozen tissue (either plain or in
embedding medium, whichever is the case) into formalin while still frozen.
Let it thaw and fix in the formalin, using a typical fixation time for a
tissue that size, then just process and embed it as you would any tissue.

> ----------
> From: 	histonet-bounces <@t> lists.utsouthwestern.edu on behalf of
Tomasz Bonda
> Sent: 	Friday, May 15, 2009 6:19 AM
> To: 	histonet <@t> lists.utsouthwestern.edu
> Subject: 	[Histonet] Embeeding frozen tissue in paraffin
> 
> Hello,
> is there any good method for embeeding fresh frozen tissue (without any
> fixation) in paraffin?
> I would be grateful for ANY suggestions.
> 
> T. Bonda
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> 
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