[Histonet] 70% alcohol fixation of brain

[TF]

Hi, i dont want to use PFA for the brain fixation (rat).
Now I tried to perfuse the rat with saline, followed with 70% alcohol. Then I do post-fixation at room temperature for 24 hours.
I also tried saline perfusion, then I directly put the whole brain into 70% alcohol.
Is this fine?

Also, for dehydration before cutting frozen sections on a cryostat/microtome, should I use 95% alcohol? I am now using 30% sucrose~

2009-05-15 



TF