[Histonet] Re: Embeeding frozen tissue in paraffin

[Hobbs, Carl]

Embedding frozen tissue in Pwax is very possible, Tomasz.
If your tissue has been frozen NOT using OCT, just place the frozen tissue into acetone that has been cooled to -20C.
x3 changes of -20C acetone ,  then immediately place tissue into molten pwax x3 changes.
Agitate frequently, gently.
I assume that your specimens are small: you will have more problems with morphology if you have BIG pieces of tissue.
I have done this with whole mouse cerebellum and the pwax H&Es looked OK...I never went further due to a job change.
(I used x3 acetone changes over 24Hrs, then x3 Pwax over a period of 3Hrs)
Very good Q...please keep experimenting and asking.
However, it is always best to give a reason for your Q, then you might  not get questions to your questions ;-)
So, why do you want to do this?

Carl.