[Histonet] Embedding Frozen Tissue in Paraffin/

[Patricia Karlisch]

I agree with Paul Monfils, there needs to be fixation prior to embedding in paraffin plus the tissue needs to be infiltrated or the paraffin will not penetrate the frozen tissue. Even if you are not looking for infiltration, the hot wax will eventually harden and the wet tissue will fall out or shrink.  Over long term storage the tissue will decay or grow lots of fungus and mold.

  


Pat Karlisch
Supervisor, Histology, Pathology and Laboratory Medicine
Penn State Milton S. Hershey Medical Center
Mail Code H179
Hershey, PA 17033
Phone (717) 531-6072
Fax: (717) 531- 7741
email: pkarlisch <@t> psu.edu

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