[Histonet] 70% alcohol fixation of brain

[APRIL SHOWERS]

> Good luck freezing a brain that is full of alcohol! Have you checked the
freezing point of alcohol?
> Why are you doing this?

I think that moire often than not, people ask before they think ;-)



On Fri, May 15, 2009 at 11:56 AM, Geoff McAuliffe <mcauliff <@t> umdnj.edu>wrote:

> Greetings TF:
>
> Good luck freezing a brain that is full of alcohol! Have you checked the
> freezing point of alcohol?
> Why are you doing this?
> Immersing a whole brain in 70% alcohol, why??
> 30% sucrose is a cryoprotectant so the brain is not full of holes from ice
> crystals.Alcohol defeats this purpose.
>
> Geoff
>
>
>
> TF wrote:
>
>> Hi, i dont want to use PFA for the brain fixation (rat).
>> Now I tried to perfuse the rat with saline, followed with 70% alcohol.
>> Then I do post-fixation at room temperature for 24 hours.
>> I also tried saline perfusion, then I directly put the whole brain into
>> 70% alcohol.
>> Is this fine?
>>
>> Also, for dehydration before cutting frozen sections on a
>> cryostat/microtome, should I use 95% alcohol? I am now using 30% sucrose~
>>
>> 2009-05-15
>>
>>
>> TF _______________________________________________
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>>
>>
>>
>
>
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> --
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> Geoff McAuliffe, Ph.D.
> Neuroscience and Cell Biology
> Robert Wood Johnson Medical School
> 675 Hoes Lane, Piscataway, NJ 08854
> voice: (732)-235-4583 mcauliff <@t> umdnj.edu
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