Re: Re: [Histonet] 70% alcohol fixation of brain

TF tifei <@t>
Fri May 15 13:14:56 CDT 2009

thanks to the reply.
some antigens will be damaged by PFA fixation and can not be retreieval.
the shock frozen section with acetone is of quite bad tissue quality. therefore I am trying alcohol!



发件人: Geoff McAuliffe 
发送时间: 2009-05-15  23:55:15 
收件人: tifei 
抄送: Histonet <@t> 
主题: Re: [Histonet] 70% alcohol fixation of brain 
Greetings TF:
Good luck freezing a brain that is full of alcohol! Have you checked the 
freezing point of alcohol?
Why are you doing this?
Immersing a whole brain in 70% alcohol, why??
30% sucrose is a cryoprotectant so the brain is not full of holes from 
ice crystals.Alcohol defeats this purpose.
TF wrote:
> Hi, i dont want to use PFA for the brain fixation (rat).
> Now I tried to perfuse the rat with saline, followed with 70% alcohol. Then I do post-fixation at room temperature for 24 hours.
> I also tried saline perfusion, then I directly put the whole brain into 70% alcohol.
> Is this fine?
> Also, for dehydration before cutting frozen sections on a cryostat/microtome, should I use 95% alcohol? I am now using 30% sucrose~
> 2009-05-15 
> TF 
> _______________________________________________
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> Histonet <@t>
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583 
mcauliff <@t>

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