Re: Re: [Histonet] 70% alcohol fixation of brain

[TF]

thanks to the reply.
some antigens will be damaged by PFA fixation and can not be retreieval.
the shock frozen section with acetone is of quite bad tissue quality. therefore I am trying alcohol!

2009-05-16 



TF 



发件人： Geoff McAuliffe 
发送时间： 2009-05-15  23:55:15 
收件人： tifei 
抄送： Histonet <@t> lists.utsouthwestern.edu 
主题： Re: [Histonet] 70% alcohol fixation of brain 
 
Greetings TF:
Good luck freezing a brain that is full of alcohol! Have you checked the 
freezing point of alcohol?
Why are you doing this?
Immersing a whole brain in 70% alcohol, why??
30% sucrose is a cryoprotectant so the brain is not full of holes from 
ice crystals.Alcohol defeats this purpose.
Geoff
TF wrote:
> Hi, i dont want to use PFA for the brain fixation (rat).
> Now I tried to perfuse the rat with saline, followed with 70% alcohol. Then I do post-fixation at room temperature for 24 hours.
> I also tried saline perfusion, then I directly put the whole brain into 70% alcohol.
> Is this fine?
>
> Also, for dehydration before cutting frozen sections on a cryostat/microtome, should I use 95% alcohol? I am now using 30% sucrose~
>
> 2009-05-15 
>
>
>
> TF 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>   
-- 
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583 
mcauliff <@t> umdnj.edu
**********************************************