[Histonet] Turnbull's Blue

[Bryan Llewellyn]

It was my understanding that there is not usually any ferrous iron that can 
be demonstrated in tissue, any of it present being securely bound up and 
masked in larger molecules.  The method used (Tirmann Schmelzer) simply 
replaces the potassium ferrocyanide of Perls' method with potassium 
ferricyanide, and ferrous iron then forms Turnbull's blue.

The control suggested is lung tissue from a miner who has inhaled iron ore 
dust, so both ferrous and ferric compounds would be expected to be present. 
Where you would get that I haven't a clue.  Possibly you could make one by 
injecting a ferrous salt solution followed by a ferric salt solution into 
fresh lung, then fixing it.  I suspect that a natural control will be 
difficult to come by.

Bryan Llewellyn


----- Original Message ----- 
From: "Wahlberg, Nikki" <Nikki.Wahlberg <@t> bsci.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, May 08, 2009 6:31 AM
Subject: RE: [Histonet] Turnbull's Blue


The study directors would like to use both the Prussian Blue and
Turnbull's Blue to demonstrate two different types of iron in the
tissue, ferric and ferrous.  We are having trouble finding a control
that contains both types of iron, thus we can not prove to the
pathologist that are stain is indeed working. We in fact are not sure if
the stain is working.

Nikki M Wahlberg
Histotechnologist II
Histo-Pathology Services
Boston Scientific
763-694-5739
wahlbern <@t> bsci.com


-----Original Message-----
From: Lee & Peggy Wenk [mailto:lpwenk <@t> sbcglobal.net]
Sent: Thursday, May 07, 2009 9:00 PM
To: Wahlberg, Nikki; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Turnbull's Blue

Turnball blue (i.e., Schmorl stain) demonstrates argentaffinic
components, such as melanin or argentaffin cells in the GI tract.

Prussian blue (i.e., the iron stain) demonstrates iron (hemosiderin).

What are you trying to demonstrate?

Peggy A. Wenk, HTL(ASCP)SLS
Beaumont Hospitals
Royal Oak, MI 48073

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Wahlberg, Nikki
Sent: Thursday, May 07, 2009 1:38 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Turnbull's Blue

Is anyone performing the Turnbull's Blue Iron stain?  If so, what type
of control do you use.  We are having a difficult time finding a good
control in order to get the stain approved in our lab.  Also, if anyone
has some tips for the procedure they would be greatly appreciated.

Thank you

Nikki M Wahlberg
Histotechnologist II
Histo-Pathology Services
Boston Scientific
763-694-5739
wahlbern <@t> bsci.com

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