[Histonet] Turnbull's Blue

[Wahlberg, Nikki]

The study directors would like to use both the Prussian Blue and
Turnbull's Blue to demonstrate two different types of iron in the
tissue, ferric and ferrous.  We are having trouble finding a control
that contains both types of iron, thus we can not prove to the
pathologist that are stain is indeed working. We in fact are not sure if
the stain is working.

Nikki M Wahlberg
Histotechnologist II
Histo-Pathology Services
Boston Scientific
763-694-5739
wahlbern <@t> bsci.com


-----Original Message-----
From: Lee & Peggy Wenk [mailto:lpwenk <@t> sbcglobal.net] 
Sent: Thursday, May 07, 2009 9:00 PM
To: Wahlberg, Nikki; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Turnbull's Blue

Turnball blue (i.e., Schmorl stain) demonstrates argentaffinic
components, such as melanin or argentaffin cells in the GI tract.

Prussian blue (i.e., the iron stain) demonstrates iron (hemosiderin).

What are you trying to demonstrate? 

Peggy A. Wenk, HTL(ASCP)SLS
Beaumont Hospitals
Royal Oak, MI 48073

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Wahlberg, Nikki
Sent: Thursday, May 07, 2009 1:38 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Turnbull's Blue

Is anyone performing the Turnbull's Blue Iron stain?  If so, what type
of control do you use.  We are having a difficult time finding a good
control in order to get the stain approved in our lab.  Also, if anyone
has some tips for the procedure they would be greatly appreciated.
 
Thank you
 
Nikki M Wahlberg
Histotechnologist II
Histo-Pathology Services
Boston Scientific
763-694-5739
wahlbern <@t> bsci.com

CONFIDENTIALITY NOTICE:  This e-mail transmission may contain
confidential or legally privileged information that is intended only for
the individual or entity named in the e-mail address.  Any unauthorized
distribution or copying of this transmittal or its attachments, if any,
is prohibited.  If you have received this e-mail transmission in error,
please reply to the sender, so that Boston Scientific Corporation can
arrange for proper delivery, then please delete the message from your
inbox.

 

 
 
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet