[Histonet] pressure cookers for IHC

Jennifer Campbell jcampbell <@t> vdxpathology.com
Mon Mar 30 09:44:10 CDT 2009


Hi everyone,
 
Does anyone use a pressure cooker for heat induced epitope retrieval?  I
know there are a lot of fancy antigen retrieval units out there that you
can buy specifically for IHC but, I know a lot of people just use a
pressure cooker with the same success.  Any specifics as to what type of
pressure cooker to use? Protocol used as far as the temp and for how
much time?  Any info would be appreciated.
 
Thanks,
 
Jen C.


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