[Histonet] Cyto prep question

Valerie Biendara cytch7 <@t> cox.net
Mon Mar 30 22:04:39 CDT 2009


This is a fairly common problem with ThinPrep slides even after educating
our clinicians.  We have found that vortexing the vial a minute or two just
before processing helps with the cellularity.   If you visually inspect the
vial there is quite a bit of clumping.  I think the vortexing helps break
apart the clumps.  The recovery rate seems to depend on how much lubricant
is present in the vial.    Vortexing is not 100% effective but it has cut
down our unsatisfactory rate.

Valerie N Biendara SCT(ASCP)IAC
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Mike Pence
Sent: Monday, March 30, 2009 9:27 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Cyto prep question

I know this many not be the forum to ask this question, but I know many
of us have cyto depts. within our labs.
 
We have been seeing an increase in the number of unsatisfactory urine
specimens from our Urology group lately and it is due to lubricant on
the ThinPrep filter.  We have worked with the Urology group and they are
using the recommended lubricant from the manufactory.  We see the
problem even when the urine is diluted in CytoLyt for several hours. Has
anyone else experienced this problem?
 
Thanks in advance,
Mike
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