[Histonet] pressure cookers for IHC

[Rosa Fields]

I am just starting to bring IHC in house to our new lab, I have been working with the tech staff at Cell Marque.  They sent me a "cuisinart" pressure cooker to work with, it is pretty nice.. has a digital timer.. gets the job done.
I have only been working up H.pylori at this time, with a pre-dilute antibody.. but I have used the pressure cooker at high temp, 15 mins with success.  It allows you to pick low/high also..
Much cheaper than other models I looked at.


Rosa Fields, HT (ASCP)
Gastroenterology Specialties
Histology Supervisor
4545 R Street
Lincoln, NE  68503
402-465-4545
rfields <@t> gidocs.net

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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jennifer Campbell
Sent: Monday, March 30, 2009 9:44 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] pressure cookers for IHC

Hi everyone,
 
Does anyone use a pressure cooker for heat induced epitope retrieval?  I
know there are a lot of fancy antigen retrieval units out there that you
can buy specifically for IHC but, I know a lot of people just use a
pressure cooker with the same success.  Any specifics as to what type of
pressure cooker to use? Protocol used as far as the temp and for how
much time?  Any info would be appreciated.
 
Thanks,
 
Jen C.
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