[Histonet] Re: Different between Richard Allan and Harris & which one is better for IHC

[Swain, Frances L]

I use Mayer's Hematoxlyin for 30-45 secs. Wash for 2 minutes, blue in Ammonia Water, wash for 5 minutes, dehydrate clear and mount if it is DAB.  If it is AEC I do the same except I rinse in distilled water before mounting with aqueous mount.

Frances L. Swain HT(ASCP) A. A. S.
Special Procedures Technician
Department of Orthopaedic Surgery
Center for Orthopaedic Research
Barton Research Building 2R28
4301 West Markham Street
Little Rock AR 72205
(501) 686-8739 PHONE
(501) 686-8987 FAX
swainfrancesl <@t> uams.edu email
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira
Sent: Friday, March 27, 2009 9:23 AM
To: ooi.ting.huay <@t> nhc.com.sg; histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: Different between Richard Allan and Harris & which one is better for IHC

Hi all, I am adding my question to the below one.

 

Which is better to use for IHC: Mayer's or just hematoxylin?

 

Thanks in advance,

Naira

 

Message: 15

Date: Fri, 27 Mar 2009 09:50:42 +0800

From: ooi.ting.huay <@t> nhc.com.sg

Subject: [Histonet] Different between Richard Allan and Harris

      Hematoxylin

 

Hi, I am interested to know what is the different between different
brands of hematoxylin especially for Richard Allan and Harris. I am glad
if there is any advice or suggestion on the choosing of hematoxylin.
Welcome any suggestion of webpage that may tell the differences too. I
am dealing with plastic resin section and doing a normal hematoxylin and
eosin staining. 

 

Your advice is appreciated. Thank you very much!

 

Regards,

Ooi

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