[Histonet] Agar removing for fluorescent stainings

[barbara.verstraeten <@t> ugent.be]

Hey everybody!

Today I tried to do a fluorescent staining on cryosectionned tissue  
embedded in agar.
I didn't see a problem in the presence of the agar untill I analyzed  
the slides under the microscope... The agar had absorbed my  
fluorescent dy!!

Does anyone know who to remove the agar where the tissue is embedded  
in to make it possible to do cryosections?
I tried with 37°C PBS to 'melt' it and rinse it away but kept sticking  
on the slide..

Any advice?

Thanks alot for your help!

Greetings,

Barbara Verstraeten, Drs.
Vertebrate Morpholgy and Developmental Biology
Department of Biology
Ghent University
K.L. Ledeganckstraat 35
B-9000 Ghent
Belgium
tel: ++32/(0)9 264 52 31
fax: ++32/(0)9 264 53 44