[Histonet] Sanderson's Rapid Bone Stain

[gayle callis]

One other counterstain can be used also.  Alizarin Red S for the calcium as
long as acid etching is not performed on the surface of thick sections.  I
would presume von Kossa could be used also if Alizarin Red S has been done
in past.  

Gayle Callis 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cathy Mayton
Sent: Thursday, March 19, 2009 2:33 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Sanderson's Rapid Bone Stain



Dear Fellow Histonetters,

There are 3 counterstains that can be used with the Sanderson's Rapid Bone
Stain (RBS).  We primarily used the acidified acid fuchsin, however, we had
clients that only used a van Gieson counterstain.  Light green can also be
used.  We normally stained for 1 minute ground sections in the RBS, rinsed
in water and then counterstained.  As Gayle mentioned, intensity of the RBS
or the counterstain can be increased with addtional time. 

Cathy Mayton

Wasatch Histo Consultants, Inc. 
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