[Histonet] Sanderson's Rapid Bone Stain

[Cathy Mayton]

Dear Fellow Histonetters,

There are 3 counterstains that can be used with the Sanderson's Rapid Bone Stain (RBS).  We primarily used the acidified acid fuchsin, however, we had clients that only used a van Gieson counterstain.  Light green can also be used.  We normally stained for 1 minute ground sections in the RBS, rinsed in water and then counterstained.  As Gayle mentioned, intensity of the RBS or the counterstain can be increased with addtional time. 

Cathy Mayton

Wasatch Histo Consultants, Inc.