[Histonet] Von Kossa staining on PMMA sections
PMonfils <@t> Lifespan.org
Thu Mar 12 10:19:49 CDT 2009
The standard Von Kossa silver stain for calcium calls for 20 minutes in the silver nitrate solution under UV light. There is a modified Von Kossa for plastic embedded bone sections, which is identical except it calls for a minimum of 6 hours in the silver nitrate solution under UV. Does anyone know why such a long staining time is recommended? Visually the calcium in the bone sections turns black within 20 minutes, so why is so much additional time needed? Thanks.
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