[Histonet] Von Kossa staining on PMMA sections

Monfils, Paul PMonfils <@t> Lifespan.org
Thu Mar 12 10:19:49 CDT 2009

The standard Von Kossa silver stain for calcium calls for 20 minutes in the silver nitrate solution under UV light.  There is a modified Von Kossa for plastic embedded bone sections, which is identical except it calls for a minimum of 6 hours in the silver nitrate solution under UV.  Does anyone know why such a long staining time is recommended?  Visually the calcium in the bone sections turns black within 20 minutes, so why is so much additional time needed?  Thanks.

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