[Histonet] RE: Von Kossa staining on PMMA sections

[Swain, Frances L]

Our Von Kossa Procedure calls for placing the slides in Silver Nitrate Solution for 2 minutes in the dark, rinse in distilled water, develop in Hydroquinone Solution for 1 minute, fix in Sodium Thiosulfate, Rinse, counterstain with Acid Fuchsin rinse in 1% Glacial Acetic Acid Solution quickly dehydrate, clear and mount.  I will be happy to send you the procedure if you would like it.  These sections are undecalcified bone embedded in MMA.  We do this procedure with GMA also.

Frances L. Swain HT(ASCP) A. A. S.
Special Procedures Technician
Department of Orthopaedic Surgery
Center for Orthopaedic Research
Barton Research Building 2R28
4301 West Markham Street
Little Rock AR 72205
(501) 686-8739 PHONE
(501) 686-8987 FAX
swainfrancesl <@t> uams.edu email
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Monfils, Paul
Sent: Thursday, March 12, 2009 10:20 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Von Kossa staining on PMMA sections

The standard Von Kossa silver stain for calcium calls for 20 minutes in the silver nitrate solution under UV light.  There is a modified Von Kossa for plastic embedded bone sections, which is identical except it calls for a minimum of 6 hours in the silver nitrate solution under UV.  Does anyone know why such a long staining time is recommended?  Visually the calcium in the bone sections turns black within 20 minutes, so why is so much additional time needed?  Thanks.
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