[Histonet] staining brain vessels

[Colleen Forster]

Again, you need to be sure that it will work in mouse samples. CD34 is 
another marker that does not work well in mouse.

Colleen Forster


anh2006 <@t> med.cornell.edu wrote:
> You could use CD31 but would need to digest first as it doesn't work well in fixed tissue without enzymatic retrieval.
>
> CD34 is a gem for brain vessels in paraffin so might work in fixed frozen as well. 
>
> Andrea
>
> ------Original Message------
> From: iskaliora
> Sender: histonet-bounces <@t> lists.utsouthwestern.edu
> To: histonet <@t> lists.utsouthwestern.edu
> Cc: ΕΛΕΝΗ ΚΟΝΣΟΛΑΚΗ
> Sent: Mar 5, 2009 5:17 AM
> Subject: [Histonet] staining brain vessels
>
> I was wondering if anybody might have an idea with the following  
> problem we are experiencing:  we want to stain for blood vessels in  
> sections of mouse brain.  Our experimental tissues have been fixed  
> overnight in 4% paraformaldehyde and have been sitting in PBS since.   
> We have tried staining with antibodies against desmin, SMA, and  
> collagen but we get NO specific signal.  We recently tried a non-fixed  
> mouse brain and got desmin to work immediately.  The problem is that  
> we need to use the fixed brains because they are our experimental  
> model and it would take too long (2 years to be exact) to generate the  
> same samples.  If anybody has come across such a problem before, or  
> has a specific protocol for vessels that works on PFA fixed brain, we  
> would appreciate the suggestions!
> thanks in advance!
> Irini
>
>
>
>
> -----------------------------------------------------
> Irini Skaliora, PhD
> Investigator C᾽ (Assistant Professor)
> Developmental Biology Division
> Biomedical Research Foundation of the Academy of Athens (BRFAA)
> Soranou Efessiou 4
> Athens 11527
> tel. +30-210-6597203 (office)
> tel. +30-210-6597482 (lab)
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> email: iskaliora <@t> bioacademy.gr
>
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