[Histonet] gelatin beads

Andrea Grantham algranth <@t> u.arizona.edu
Thu Mar 5 09:19:53 CST 2009


Good Morning,
I have an investigator who is bringing me rat brains that have been 
injected with gelatin beads. I'm sectioning through the injection 
site(s) and she is hoping to see the beads. BUT even though the 
injection sites are easy to see there are no gelatin beads present. 
My question is, are they being washed out somehow by 
fixation/processing or dropping out in the waterbath as she is 
telling me? Or are they melting in the paraffin steps (60 degrees)? 
If either of these are the case I'm thinking that frozen sections 
might be the solution?
Sorry I don't have more info on the beads. I'll try to get that today.
Thanks,
Andi
.....................................................................
: Andrea Grantham, HT(ASCP)     Dept. of Cell Biology & Anatomy     :
: Sr. Research Specialist       University of Arizona               :
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: (FAX:  520-626-2097)          (email:  algranth <@t> u.arizona.edu)       :
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