[Histonet] gelatin beads

[Rene J Buesa]

The gelatin beads are water soluble and will dissolve in any water containing step. Also, as you point out, they will melt at high temperature.
Your solution of doing FS is probably the best way of trying to look for these beads.
René J.

--- On Thu, 3/5/09, Andrea Grantham <algranth <@t> u.arizona.edu> wrote:

From: Andrea Grantham <algranth <@t> u.arizona.edu>
Subject: [Histonet] gelatin beads
To: histonet <@t> lists.utsouthwestern.edu
Date: Thursday, March 5, 2009, 10:19 AM

Good Morning,
I have an investigator who is bringing me rat brains that have been injected
with gelatin beads. I'm sectioning through the injection site(s) and she is
hoping to see the beads. BUT even though the injection sites are easy to see
there are no gelatin beads present. My question is, are they being washed out
somehow by fixation/processing or dropping out in the waterbath as she is
telling me? Or are they melting in the paraffin steps (60 degrees)? If either of
these are the case I'm thinking that frozen sections might be the solution?
Sorry I don't have more info on the beads. I'll try to get that today.
Thanks,
Andi
.....................................................................
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