[Histonet] problem eyes

[Maxim Peshkov]

We also addes 4-5 drops of Tween 20 in waterbath.
Human eye's sections have not any wrinlkes.
Maxim Peshkov,
Russia,
Taganrog.

--- On Wed, 3/4/09, Perry, Margaret
> From: Perry, Margaret <Margaret.Perry <@t> sdstate.edu>
> Subject: [Histonet] problem eyes
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Date: Wednesday, March 4, 2009, 4:04 PM
>
> Please help!  We have been trying to cut whole eyes
> from a pig.  They need to be
> nice enough for a publication.  We are having the
> devil of a time because no
> matter what we do there are wrinkles.  If we turn
> the waterbath up to stretch
> things out the retina detaches.  Do any of you have suggestions?  We uses
> Surgipath  EM400 for embedding paraffin and and
> their infiltration medium.  Is
> this to soft? The lowest we can turn our waterbath is 38 degrees C.
>
> Margaret Perry HT (ASCP)
> IHC Lab Manager Veterinary Science
> Animal Disease Research and Diagnostic Lab
> South Dakota State University
> Box 2175 North Campus Drive
> Brookings SD 57007.