[Histonet] problem eyes

[Tony Henwood]

Doesn't help with the wrinkles in OLD large Histotechs.

Sorry, couldn't resist

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Va Paula Sicurello
Sent: Thursday, 5 March 2009 8:23 AM
To: histonet <@t> lists.utsouthwestern.edu; MargaretPerry; rjbuesa <@t> yahoo.com
Subject: Re: [Histonet] problem eyes



Really?  Liquid laundry soap?  Does this help with all wrinkles in large specimens?


Paula Sicurello
VA Medical Center San Diego
Veterans Medical Research Foundation (VMRF) 
Core Microscope Facility, room B141
3350 La Jolla Village Dr., MC151
San Diego, CA 92161
858-552-8585 x2397


--- On Wed, 3/4/09, Rene J Buesa <rjbuesa <@t> yahoo.com> wrote:

> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> Subject: Re: [Histonet] problem eyes
> To: "histonet <@t> lists.utsouthwestern.edu" 
> <histonet <@t> lists.utsouthwestern.edu>, "Perry, Margaret" <Margaret.Perry <@t> sdstate.edu>
> Date: Wednesday, March 4, 2009, 9:14 PM
> Add a few drops (4-5) of liquid
> detergent, but not dishwasher detergent (because it will
> dissolve the paraffin).
> rené J.
> 
> --- On Wed, 3/4/09, Perry, Margaret <Margaret.Perry <@t> sdstate.edu>
> wrote:
> 
> From: Perry, Margaret <Margaret.Perry <@t> sdstate.edu>
> Subject: [Histonet] problem eyes
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Date: Wednesday, March 4, 2009, 4:04 PM
> 
> Please help!  We have been trying to cut whole eyes
> from a pig.  They need to be
> nice enough for a publication.  We are having the
> devil of a time because no
> matter what we do there are wrinkles.  If we turn the waterbath up to 
> stretch things out the retina detaches.  Do any of you have
> suggestions?  We uses
> Surgipath  EM400 for embedding paraffin and and their
> infiltration medium.  Is
> this to soft? The lowest we can turn our waterbath is 38
> degrees C.
> 
> Margaret Perry HT (ASCP)
> IHC Lab Manager Veterinary Science
> Animal Disease Research and Diagnostic Lab
> South Dakota State University
> Box 2175 North Campus Drive
> Brookings SD 57007
> 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu 
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu 
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 


      


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

*********************************************************************
This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender.

Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead

This note also confirms that this email message has been
virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses.
**********************************************************************