[Histonet] problem eyes

[Merced Leiker]

Good question! I'd like to know that, too! But with any size specimen, 
please!

--On Wednesday, March 04, 2009 1:23 PM -0800 Va Paula Sicurello 
<vapatpxs <@t> yahoo.com> wrote:

>
> Really?  Liquid laundry soap?  Does this help with all wrinkles in large
> specimens?
>
>
> Paula Sicurello
> VA Medical Center San Diego
> Veterans Medical Research Foundation (VMRF)
> Core Microscope Facility, room B141
> 3350 La Jolla Village Dr., MC151
> San Diego, CA 92161
> 858-552-8585 x2397
>
>
> --- On Wed, 3/4/09, Rene J Buesa <rjbuesa <@t> yahoo.com> wrote:
>
>> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
>> Subject: Re: [Histonet] problem eyes
>> To: "histonet <@t> lists.utsouthwestern.edu"
>> <histonet <@t> lists.utsouthwestern.edu>, "Perry, Margaret"
>> <Margaret.Perry <@t> sdstate.edu> Date: Wednesday, March 4, 2009, 9:14 PM
>> Add a few drops (4-5) of liquid
>> detergent, but not dishwasher detergent (because it will
>> dissolve the paraffin).
>> rené J.
>>
>> --- On Wed, 3/4/09, Perry, Margaret <Margaret.Perry <@t> sdstate.edu>
>> wrote:
>>
>> From: Perry, Margaret <Margaret.Perry <@t> sdstate.edu>
>> Subject: [Histonet] problem eyes
>> To: "histonet <@t> lists.utsouthwestern.edu"
>> <histonet <@t> lists.utsouthwestern.edu>
>> Date: Wednesday, March 4, 2009, 4:04 PM
>>
>> Please help!  We have been trying to cut whole eyes
>> from a pig.  They need to be
>> nice enough for a publication.  We are having the
>> devil of a time because no
>> matter what we do there are wrinkles.  If we turn the
>> waterbath up to stretch
>> things out the retina detaches.  Do any of you have
>> suggestions?  We uses
>> Surgipath  EM400 for embedding paraffin and and their
>> infiltration medium.  Is
>> this to soft? The lowest we can turn our waterbath is 38
>> degrees C.
>>
>> Margaret Perry HT (ASCP)
>> IHC Lab Manager Veterinary Science
>> Animal Disease Research and Diagnostic Lab
>> South Dakota State University
>> Box 2175 North Campus Drive
>> Brookings SD 57007
>>
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Merced M Leiker
Research Technician II
354 Biomedical Research Building
School of Medicine and Biomedical Sciences
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214
Ph: (716) 829-6033
Fx: (716) 829-2725

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