[Histonet] problem eyes

[Rene J Buesa]

Add a few drops (4-5) of liquid detergent, but not dishwasher detergent (because it will dissolve the paraffin).
rené J.

--- On Wed, 3/4/09, Perry, Margaret <Margaret.Perry <@t> sdstate.edu> wrote:

From: Perry, Margaret <Margaret.Perry <@t> sdstate.edu>
Subject: [Histonet] problem eyes
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Date: Wednesday, March 4, 2009, 4:04 PM

Please help!  We have been trying to cut whole eyes from a pig.  They need to be
nice enough for a publication.  We are having the devil of a time because no
matter what we do there are wrinkles.  If we turn the waterbath up to stretch
things out the retina detaches.  Do any of you have suggestions?  We uses
Surgipath  EM400 for embedding paraffin and and their infiltration medium.  Is
this to soft? The lowest we can turn our waterbath is 38 degrees C.

Margaret Perry HT (ASCP)
IHC Lab Manager Veterinary Science
Animal Disease Research and Diagnostic Lab
South Dakota State University
Box 2175 North Campus Drive
Brookings SD 57007

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