[Histonet] problem eyes

Wed Mar 4 15:04:23 CST 2009

Please help!  We have been trying to cut whole eyes from a pig.  They need to be nice enough for a publication.  We are having the devil of a time because no matter what we do there are wrinkles.  If we turn the waterbath up to stretch things out the retina detaches.  Do any of you have suggestions?  We uses Surgipath  EM400 for embedding paraffin and and their infiltration medium.  Is this to soft? The lowest we can turn our waterbath is 38 degrees C.

Margaret Perry HT (ASCP)
IHC Lab Manager Veterinary Science
Animal Disease Research and Diagnostic Lab
South Dakota State University
Box 2175 North Campus Drive
Brookings SD 57007