[Histonet] Section thickness

[Vanessa J. Phelan]

Ammunition...no, was more just inquiring what the what the average would be
and if there was any specific reason for thinner sections other that just
preference.

Thanks

Vanessa



On 3/3/09 9:37 AM, "Rene J Buesa" <rjbuesa <@t> yahoo.com> wrote:

> Vanessa:
> Lymph nodes for cellular details (special request) = 3 µm
> H&E and all other special procedures = 5 µm
> Sections for bone marrow and liver reticulum stain = 7 µm
> Brain and central nervous system = 10 µm
>  
> Now a question, why do you want to know this? To have "ammunition" to
> challenge what is done in your new lab? Not a wise move. I don't think that
> they would mind if you cut thinner, but they will mind if you start bringing
> this issue about. Let your thinner sections "speak for themselves". It will
> get the moment that "by example" your way will prevail.
> René J.
> 
> --- On Tue, 3/3/09, Vanessa J. Phelan <vjp2105 <@t> columbia.edu> wrote:
>> From: Vanessa J. Phelan <vjp2105 <@t> columbia.edu>
>> Subject: [Histonet] Section thickness
>> To: histonet <@t> lists.utsouthwestern.edu
>> Date: Tuesday, March 3, 2009, 9:14 AM
>> 
>> Hi Guys,
>> 
>> Just wondering what thickness you cut sections at? I was always used to
>> cutting at 2-3 microns in my last lab, however in my new place they are
>> cutting at 6 microns (for both H & Es and IHC), which seems to me as really
>> quite thick! What would be the average cutting thickness?
>> 
>> Thanks a mill,
>> 
>> Vanessa 
>> 
>> 
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>