[Histonet] Good Perfusion--I stand corrected.

[JR R]

I stand correct on the perfusion under anesthesia issue.

Jerry Ricks
Research Scientist
University of Washington
Department of Pathology


Date: Tue, 30 Jun 2009 12:37:20 -0700
Subject: Re: [Histonet] Good Perfusion...
From: chana.de.wolf <@t> gmail.com
To: jfish <@t> gladstone.ucsf.edu
CC: rosenfeldtek <@t> hotmail.com; histonet <@t> lists.utsouthwestern.edu

Histonetters,

Perfusion under deep anesthesia is most certainly
not unethical NOR illegal, and, in fact (as mentioned by Jo Dee), it is
necessary for optimal perfusion and fixation -- the intracellular
ischemic cascade begins immediately upon circulatory arrest, setting
off a chain of events highly detrimental to subsequent perfusion.
Indeed, ischemia quickly leads to the "no-reflow" phenomenon,
effectively guaranteeing that you are not perfusing all tissues
adequately at all! It is therefore *most* beneficial to begin perfusion
with a beating heart, and every perfusion protocol I have ever worked
under requires it (especially if the tissues are to be used for EM). 


I perform around 5-10 perfusions per week *specifically* to study
the effects of ischemia on reperfusion and neural ultrastructure. 

Of
course, you should check with your institution's particular rules and
regulations, but perfusion begun under anesthesia is scientifically
justified for the reasons mentioned above and should therefore be
relatively easy to have approved by your IACUC.


Sincerely,

Chana de Wolf

On Tue, Jun 30, 2009 at 11:37 AM, Jo Dee Fish <jfish <@t> gladstone.ucsf.edu> wrote:

Dear Jerry and histonetters,



I don't believe this is unethical or illegal.  It is written into our IACUC

approved protocols as follows:



Chemical Method of Euthanasia:  "Perfusion under general anesthesia, Avertin

or Halothane induced.  Bilateral thoracotomy."



Could it only be forbidden by your facility?



I wonder if other facilities have rules against such things.  But we insist

that our investigators use perfusion under anesthsia because the deeper

tissues are much better fixed and blood removal is more thorough and

complete by this method.



Jo Dee





~~Jo Dee Fish~~

Senior Research Technologist

The J. David Gladstone Institutes

Co-manager Histology and Microscopy Core



Telephone: (415) 734-2567

Fax: (415) 355-0824

E-mail: jfish <@t> gladstone.ucsf.edu



Mailing address:

The J. David Gladstone Institutes

1650 Owens Street

San Francisco, CA 94158



-----Original Message-----

From: histonet-bounces <@t> lists.utsouthwestern.edu

[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of JR R

Sent: Tuesday, June 30, 2009 10:49 AM

To: histonet <@t> lists.utsouthwestern.edu

Subject: [Histonet] Good Perfusion...





No, I wouldn't nick the atrium.



Instead, cut the femoral artery at the groin.



And of course, you cannot perfuse a living, anesthetized animal.  That would

be unethical and also illegal.



Jerry Ricks

Research Scientist

University of Washington

Department of Pathology



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