[Histonet] signs of good perfusion

[Merced M Leiker]

i think it probably would be better; but good luck getting approval to do 
that.


--On Tuesday, June 30, 2009 9:32 AM +0530 raghul 
<jayaprakash.raghul <@t> rccltd.in> wrote:

> Dear Dzung,
>
> I wonder whether the perfusion would be good in an euthanized animal with
> the circulation arrested. It would be better to try it under deep
> anesthesia. Certainly perfusion would be better
>
> Regards
> Raghul
> RCC laboratories India Pvt. Ltd
>
>
> Hi Everyone!!
>
>    I am perfusing CO2 euthanized 3 weeks old mice with PBS only.  I am
> nicking the upper right atrium of heart to collect the gushed out blood
> and then perfusing through ventricle using a 21G butterfly needle and
> peristaltic pump.  Sometimes I see the lungs swelling up and fluid comes
> out of mouth.  Occasionally, I see the liver fade to light pink.  Most of
> the time the paws become white.  But the brain and spinal cord (my
> tissues of interest) are always white, and seem to have been perfused.  I
> was wondering how to improve this to get more consistent good perfusions,
> and what signs should I look for to indicate good perfusion?
>
> Thanks much,
>
> Dzung
> NIAID
>
>
>
>
> ------------------------------
>
> Message: 5
> Date: Mon, 29 Jun 2009 08:47:07 -0700 (PDT)
> From: Phyllis Thaxton <dchihc <@t> yahoo.com>
> Subject: [Histonet] Cardboard Paraffin Catchers
> To: Histonet <@t> lists.utsouthwestern.edu
> Message-ID: <372922.65853.qm <@t> web43506.mail.sp1.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Does anyone remember the little cardboard microtome trays for paraffin
> waste? If so please email me the company that makes them.
>  Phyllis Thaxton HT(ASCP)QIHC
> DCH Regional Medical Center
> Tuscaloosa, AL
>
>
>
>
> ------------------------------
>
> Message: 6
> Date: Mon, 29 Jun 2009 12:07:04 -0400
> From: Merced M Leiker <leiker <@t> buffalo.edu>
> Subject: Re: [Histonet] Signs of good perfusion
> To: "Thach, Dzung (NIH/NIAID) [E]" <thachdc <@t> niaid.nih.gov>,
> 	histonet <@t> lists.utsouthwestern.edu
> Message-ID: <1D8E958050ABAD54BD852005 <@t> CDYwxp1931.ad.med.buffalo.edu>
> Content-Type: text/plain; charset=us-ascii; format=flowed
>
> Live going pale is a good sign, your tissues of interest going pale is an
> even better sign, but fluid coming out of the mouth (or even the nose, or
> additionally, any kind of bloating or swelling in the animal) is a bad
> sign. You may not be able to get good perfusion (pale tissues) if this
> happens before your tissues turn pale, as the pressure is too high
> causing  fluid to leak out of the vasculature....ideally you want to push
> the blood  out through the the hole you made in the right atrium, not
> through the  walls of the vessels. at what rate do you perfuse? if this
> happens a lot  slow it down.
>
> Hope this helps.
>
> --On Monday, June 29, 2009 11:31 AM -0400 "Thach, Dzung (NIH/NIAID) [E]"
> <thachdc <@t> niaid.nih.gov> wrote:
>
>> Hi Everyone!!
>>
>>    I am perfusing CO2 euthanized 3 weeks old mice with PBS only.  I am
>> nicking the upper right atrium of heart to collect the gushed out blood
>> and then perfusing through ventricle using a 21G butterfly needle and
>> peristaltic pump.  Sometimes I see the lungs swelling up and fluid comes
>> out of mouth.  Occasionally, I see the liver fade to light pink.  Most of
>> the time the paws become white.  But the brain and spinal cord (my
>> tissues of interest) are always white, and seem to have been perfused.  I
>> was wondering how to improve this to get more consistent good perfusions,
>> and what signs should I look for to indicate good perfusion?
>>
>> Thanks much,
>>
>> Dzung
>> NIAID
>>
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>
>
>
> Merced M Leiker
> Research Technician II
> Cardiovascular Medicine
> 348 Biomedical Research Building
> State University of New York at Buffalo
> 3435 Main St, Buffalo, NY  14214
> leiker <@t> buffalo.edu
> 716-829-6118 (Ph)
> 716-829-2665 (Fx)
>
> No trees were harmed in the sending of this email.
> However, many electrons were severely inconvenienced.
>
>
>
>
> ------------------------------
>
> Message: 7
> Date: Mon, 29 Jun 2009 09:39:02 -0700 (PDT)
> From: Steven Coakley <sjchtascp <@t> yahoo.com>
> Subject: [Histonet] WI/MI
> To: Histonet <@t> lists.utsouthwestern.edu
> Message-ID: <385293.93112.qm <@t> web38204.mail.mud.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> I'm looking for a few techs from Northern WI or Northern MI to let me know
> when HT positions become availiable.
>
> Thanks
>
>
>
>
> ------------------------------
>
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> End of Histonet Digest, Vol 67, Issue 32
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Merced M Leiker
Research Technician II
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY  14214
leiker <@t> buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.