[Histonet] signs of good perfusion

[raghul]

Dear Dzung,

I wonder whether the perfusion would be good in an euthanized animal with
the circulation arrested. It would be better to try it under deep
anesthesia. Certainly perfusion would be better

Regards
Raghul
RCC laboratories India Pvt. Ltd


Hi Everyone!!

   I am perfusing CO2 euthanized 3 weeks old mice with PBS only.  I am
nicking the upper right atrium of heart to collect the gushed out blood and
then perfusing through ventricle using a 21G butterfly needle and
peristaltic pump.  Sometimes I see the lungs swelling up and fluid comes out
of mouth.  Occasionally, I see the liver fade to light pink.  Most of the
time the paws become white.  But the brain and spinal cord (my tissues of
interest) are always white, and seem to have been perfused.  I was wondering
how to improve this to get more consistent good perfusions, and what signs
should I look for to indicate good perfusion?

Thanks much,

Dzung
NIAID




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Message: 5
Date: Mon, 29 Jun 2009 08:47:07 -0700 (PDT)
From: Phyllis Thaxton <dchihc <@t> yahoo.com>
Subject: [Histonet] Cardboard Paraffin Catchers
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <372922.65853.qm <@t> web43506.mail.sp1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Does anyone remember the little cardboard microtome trays for paraffin
waste? If so please email me the company that makes them.
 Phyllis Thaxton HT(ASCP)QIHC
DCH Regional Medical Center
Tuscaloosa, AL 


      

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Message: 6
Date: Mon, 29 Jun 2009 12:07:04 -0400
From: Merced M Leiker <leiker <@t> buffalo.edu>
Subject: Re: [Histonet] Signs of good perfusion
To: "Thach, Dzung (NIH/NIAID) [E]" <thachdc <@t> niaid.nih.gov>,
	histonet <@t> lists.utsouthwestern.edu
Message-ID: <1D8E958050ABAD54BD852005 <@t> CDYwxp1931.ad.med.buffalo.edu>
Content-Type: text/plain; charset=us-ascii; format=flowed

Live going pale is a good sign, your tissues of interest going pale is an 
even better sign, but fluid coming out of the mouth (or even the nose, or 
additionally, any kind of bloating or swelling in the animal) is a bad 
sign. You may not be able to get good perfusion (pale tissues) if this 
happens before your tissues turn pale, as the pressure is too high causing 
fluid to leak out of the vasculature....ideally you want to push the blood 
out through the the hole you made in the right atrium, not through the 
walls of the vessels. at what rate do you perfuse? if this happens a lot 
slow it down.

Hope this helps.

--On Monday, June 29, 2009 11:31 AM -0400 "Thach, Dzung (NIH/NIAID) [E]" 
<thachdc <@t> niaid.nih.gov> wrote:

> Hi Everyone!!
>
>    I am perfusing CO2 euthanized 3 weeks old mice with PBS only.  I am
> nicking the upper right atrium of heart to collect the gushed out blood
> and then perfusing through ventricle using a 21G butterfly needle and
> peristaltic pump.  Sometimes I see the lungs swelling up and fluid comes
> out of mouth.  Occasionally, I see the liver fade to light pink.  Most of
> the time the paws become white.  But the brain and spinal cord (my
> tissues of interest) are always white, and seem to have been perfused.  I
> was wondering how to improve this to get more consistent good perfusions,
> and what signs should I look for to indicate good perfusion?
>
> Thanks much,
>
> Dzung
> NIAID
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



Merced M Leiker
Research Technician II
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY  14214
leiker <@t> buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.




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Message: 7
Date: Mon, 29 Jun 2009 09:39:02 -0700 (PDT)
From: Steven Coakley <sjchtascp <@t> yahoo.com>
Subject: [Histonet] WI/MI
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <385293.93112.qm <@t> web38204.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

I'm looking for a few techs from Northern WI or Northern MI to let me know
when HT positions become availiable.
 
Thanks


      

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End of Histonet Digest, Vol 67, Issue 32
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