[Histonet] Signs of good perfusion

[Thach, Dzung (NIH/NIAID) [E]]

Hi Everyone!!

   I am perfusing CO2 euthanized 3 weeks old mice with PBS only.  I am
nicking the upper right atrium of heart to collect the gushed out blood and
then perfusing through ventricle using a 21G butterfly needle and
peristaltic pump.  Sometimes I see the lungs swelling up and fluid comes out
of mouth.  Occasionally, I see the liver fade to light pink.  Most of the
time the paws become white.  But the brain and spinal cord (my tissues of
interest) are always white, and seem to have been perfused.  I was wondering
how to improve this to get more consistent good perfusions, and what signs
should I look for to indicate good perfusion?

Thanks much,

Dzung
NIAID