[Histonet] Millipore MAB1281 anti human nuclei IHC on FFPE

PALMER Jason (SVHM) Jason.PALMER <@t> svhm.org.au
Mon Jun 29 01:36:12 CDT 2009

Hi all.

Just wondering if anyone has managed to use Millipore mouse anti human nuclei antibody (clone 235-1, MAB1281) successfully with immunostaining of FFPE tissues??  The datasheet says it is possible, at 1:20 and with citrate retrieval but I have had no luck with this on a variety of human tissues, using a standard ABC with DAB protocol. Also no luck with proteinase K.  (There is also an IHC world protocol which uses 1:20 with citrate.)  I see some apparent labelling of epidermal nuclei, for example, maybe half of all nuclei , but also see the same thing in diluent-only negative controls, so clearly the staining seen is not specific.

Thanks for any help,


Jason Palmer
Histology Laboratory Coordinator
Bernard O'Brien Institute
42 Fitzroy St, Fitzroy Victoria 3065
tel +61 3 9288 4018
fax +61 3 9416 0926
email: jason.palmer <@t> svhm.org.au

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