[Histonet] Millipore MAB1281 anti human nuclei IHC on FFPE

[PALMER Jason (SVHM)]

Hi all.

Just wondering if anyone has managed to use Millipore mouse anti human nuclei antibody (clone 235-1, MAB1281) successfully with immunostaining of FFPE tissues??  The datasheet says it is possible, at 1:20 and with citrate retrieval but I have had no luck with this on a variety of human tissues, using a standard ABC with DAB protocol. Also no luck with proteinase K.  (There is also an IHC world protocol which uses 1:20 with citrate.)  I see some apparent labelling of epidermal nuclei, for example, maybe half of all nuclei , but also see the same thing in diluent-only negative controls, so clearly the staining seen is not specific.

Thanks for any help,

Jason

Jason Palmer
Histology Laboratory Coordinator
Bernard O'Brien Institute
42 Fitzroy St, Fitzroy Victoria 3065
Australia
tel +61 3 9288 4018
fax +61 3 9416 0926
email: jason.palmer <@t> svhm.org.au

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