FW: [Histonet] xylene and frozen sections

[Lynn Dike]

Lynn


 



From: lynnd01 <@t> hotmail.com
To: lynnd01 <@t> hotmail.com
Subject: RE: [Histonet] xylene and frozen sections
Date: Thu, 18 Jun 2009 19:53:52 -0500



Is there any literature that says xylene will fade progressive staining H&E (using Surgipath Harris Hematoxylin) over a period of years?  Our frozen staining series after the eosin has two 95% ROH's and six 100% and then clear in Propar.  I mistakenly replaced the last three 100% ROH with xylene when I rotated the series.  I'm told that anyone who knows anything about chemistry knows this is a big deal and the sections will fade.  Every lab I've worked in used xylene to clear frozen sections.  I understand that I messed up because this isn't our proceedure, but I don't believe I sacrificed patient care.  Am I wrong?


Lynn


  

> From: lynnd01 <@t> hotmail.com
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Thu, 18 Jun 2009 19:47:14 -0500
> Subject: [Histonet] xylene and frozen sections
> 
> 
> 
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> 
> Lynn
> 
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