[Histonet] Superfrost plus gold slides vs superfrost plus

[Susan Bachus]

Good old fashioned gelatin subbing is very cheap & works great--I have never 
seen tissue separate from a subbed slide!   Susan
----- Original Message ----- 
From: "Peggy Bisher" <mbisher <@t> Princeton.EDU>
To: "Milne, Katy" <kmilne <@t> bccancer.bc.ca>; 
<histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, June 19, 2009 3:34 PM
Subject: Re: [Histonet] Superfrost plus gold slides vs superfrost plus


>I had a group here who were having trouble with their cryo-sections 
>sticking
> to the slides. Their samples were rat brains. He found that the Gold Super
> Frost Plus worked well with in-situ but not for immuno. At least that is 
> the
> comment he wrote down and put on our bulletin board in the lab. Most of 
> the
> people in the lab tend to use the Super Frost Plus (not the Gold) and they
> seem pretty happy with them.
>
>
> Margaret E. Bisher
> Electron Microscopy & Histology Core Facility Manager
> Department of Molecular Biology
> Princeton University
> Moffett Laboratory, Room 113
> Princeton, New Jersey
> Office: (609) 258-7026
> Fax: (609) 258-8468
> mbisher <@t> princeton.edu
>
>
>
>
>
> On 6/19/09 4:26 PM, "Milne, Katy" <kmilne <@t> bccancer.bc.ca> wrote:
>
>> Hi everyone,
>>
>> I'm curious if anyone has tried using Superfrost plus gold slides, are
>> they any better than the regular superfrost plus?  I'm dealing with
>> frozen lymph node on a Ventana discovery, I've tried a few different
>> fixing/drying combinations but I still have tissue lifting (not
>> completely but enough to be bothersome!).  Wondering if it's worth
>> giving the gold slides a go.
>>
>> Thanks,
>> Katy
>>
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>
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