[Histonet] Superfrost plus gold slides vs superfrost plus

[Maria Katleba]

But doesn't the gelatin's protein interfere with immuno results? That's why we do not use glue, gelatin, or any adhesive on immuno cases.

Maria Katleba HT (ASCP), MS, MBA
Pathology Dept. Mgr
Queen of the Valley Medical Center
Pathology Laboratory
Napa Ca

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Susan Bachus
Sent: Friday, June 19, 2009 1:42 PM
To: Peggy Bisher; Milne, Katy; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Superfrost plus gold slides vs superfrost plus

Good old fashioned gelatin subbing is very cheap & works great--I have never
seen tissue separate from a subbed slide!   Susan
----- Original Message -----
From: "Peggy Bisher" <mbisher <@t> Princeton.EDU>
To: "Milne, Katy" <kmilne <@t> bccancer.bc.ca>;
<histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, June 19, 2009 3:34 PM
Subject: Re: [Histonet] Superfrost plus gold slides vs superfrost plus


>I had a group here who were having trouble with their cryo-sections
>sticking
> to the slides. Their samples were rat brains. He found that the Gold Super
> Frost Plus worked well with in-situ but not for immuno. At least that is
> the
> comment he wrote down and put on our bulletin board in the lab. Most of
> the
> people in the lab tend to use the Super Frost Plus (not the Gold) and they
> seem pretty happy with them.
>
>
> Margaret E. Bisher
> Electron Microscopy & Histology Core Facility Manager
> Department of Molecular Biology
> Princeton University
> Moffett Laboratory, Room 113
> Princeton, New Jersey
> Office: (609) 258-7026
> Fax: (609) 258-8468
> mbisher <@t> princeton.edu
>
>
>
>
>
> On 6/19/09 4:26 PM, "Milne, Katy" <kmilne <@t> bccancer.bc.ca> wrote:
>
>> Hi everyone,
>>
>> I'm curious if anyone has tried using Superfrost plus gold slides, are
>> they any better than the regular superfrost plus?  I'm dealing with
>> frozen lymph node on a Ventana discovery, I've tried a few different
>> fixing/drying combinations but I still have tissue lifting (not
>> completely but enough to be bothersome!).  Wondering if it's worth
>> giving the gold slides a go.
>>
>> Thanks,
>> Katy
>>
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>
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