[Histonet] biocare bcl 6

[Orr, Rebecca]

Message: 3
Date: Mon, 15 Jun 2009 15:34:33 -0400
From: "Justin Peters" <JPeters <@t> bostwicklaboratories.com>
Subject: [Histonet] BCL-6
To: <histonet <@t> lists.utsouthwestern.edu>
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I am having trouble getting bcl-6 antibody to work (Biocare CM223).
Does anyone have a good protocol for me to try?

Hi Justin,
I run BCl-6 from concentrate 1:50 with Biocare's RED diluent with a Decloaker using RED diluent.
Most antibodies perform well with the "green" diluent; this particular antibody is best using the "RED".

I also use Mach4 detection...  if problems persist, contact me offline, I may be able to help with some other suggestions.
Hope this helps.

Becky Orr CLA,HT(ASCP)QIHC
Technical Specialist
Anatomic Pathology
NorthShore University HealthSystem
847-570-2771

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