[Histonet] biocare bcl 6

Orr, Rebecca ROrr <@t> northshore.org
Tue Jun 16 12:29:47 CDT 2009

Message: 3
Date: Mon, 15 Jun 2009 15:34:33 -0400
From: "Justin Peters" <JPeters <@t> bostwicklaboratories.com>
Subject: [Histonet] BCL-6
To: <histonet <@t> lists.utsouthwestern.edu>
        <24D22DE9E488AA43BF92A4389F2DDB1F05DFAF54 <@t> mail1.BOSTWICK.COM>
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I am having trouble getting bcl-6 antibody to work (Biocare CM223).
Does anyone have a good protocol for me to try?

Hi Justin,
I run BCl-6 from concentrate 1:50 with Biocare's RED diluent with a Decloaker using RED diluent.
Most antibodies perform well with the "green" diluent; this particular antibody is best using the "RED".

I also use Mach4 detection...  if problems persist, contact me offline, I may be able to help with some other suggestions.
Hope this helps.

Technical Specialist
Anatomic Pathology
NorthShore University HealthSystem


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