[Histonet] GMS-Fungus on nails

[Patsy Ruegg]

For really difficult tissues such as bone, cartilage and perhaps nails I
make a 5% solution of elmers glue in dih20 and dip regular uncoated slides
in the glue, let them air dry.  Use the glue coated slides to pick up
sections, just dipping in the water bath seems to activate the glue enough
to help the sections stay on during staining.  Dry the sections picked up on
the glue coated slides in a 60dc oven overnight if possible or I use a slide
warmer and lay them flat and dry at 55dc (that is as high as my slides
warmer goes) for several hours.

Good luck,
Patsy


Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
email pruegg <@t> ihctech.net
website www.ihctech.net
IHC Resource Group www.ihcrg.org 


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Clare
Thornton
Sent: Friday, July 24, 2009 9:31 AM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] GMS-Fungus on nails

We do a GMS for fungus on all nails we receive.  We often have a difficult
time keeping the nail tissue on the slide.  We've tried baking for long
periods, pre-treating in formalin, using silane slides, with no luck.  Even
when the nails cut relatively easily we still lose it, and end up running
several GMS stains before we might get a speck or two of tissue we can look
at.  We use the Artisan stainer for our specials.  We are really not
interested in performing the GMS manually, due to volume and turn around
time restrictions.  Any suggestions?

Clare J. Thornton, HTL(ASCP)
Assistant Histology Supervisor
Dahl-Chase Diagnostic Services
417 State Street, Suite 540
Bangor, ME 04401
cthornton <@t> dahlchase.com

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