[Histonet] GMS-Fungus on nails

[Donella Stillings]

We use Albumin Solution from Sigma (A7034-10ml) on our slides. We stain
on the Artisan and or the Ventana stainer as well.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Clare
Thornton
Sent: Friday, July 24, 2009 9:31 AM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] GMS-Fungus on nails

We do a GMS for fungus on all nails we receive.  We often have a
difficult time keeping the nail tissue on the slide.  We've tried baking
for long periods, pre-treating in formalin, using silane slides, with no
luck.  Even when the nails cut relatively easily we still lose it, and
end up running several GMS stains before we might get a speck or two of
tissue we can look at.  We use the Artisan stainer for our specials.  We
are really not interested in performing the GMS manually, due to volume
and turn around time restrictions.  Any suggestions?

Clare J. Thornton, HTL(ASCP)
Assistant Histology Supervisor
Dahl-Chase Diagnostic Services
417 State Street, Suite 540
Bangor, ME 04401
cthornton <@t> dahlchase.com

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