[Histonet] GMS-Fungus on nails

[Joe Nocito]

We soak our nails in 20% sodium hydroxide for at least an hour, rinse in 
running tap water for a few minutes before  we place the cassettes on the 
tissue processor. We use positive charged slides, heat slides in 80 degree 
oven for 20 minutes and perform a PAS/fungus on the slides. The PAS is a lot 
more gentler on the tissue that the GMS. We are the toenail lab for the U.S. 
Air Force ,

Joe "The Toe"

----- Original Message ----- 
From: "jstaruk" <jstaruk <@t> masshistology.com>
To: "'Clare Thornton'" <CThornton <@t> dahlchase.com>; 
<histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, July 24, 2009 10:41 AM
Subject: RE: [Histonet] GMS-Fungus on nails


> Prepare a 10% solution of Titebond II premium wood glue (found in most
> hardware stores).  Dip the slide in the solution just before mounting the
> section on the slide.  Let the slide dry and stain away.
>
> Jim
>
> _______________________
> James E. Staruk HT(ASCP)
> www.masshistology.com
>   www.nehorselabs.com
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Clare
> Thornton
> Sent: Friday, July 24, 2009 11:31 AM
> To: 'histonet <@t> lists.utsouthwestern.edu'
> Subject: [Histonet] GMS-Fungus on nails
>
> We do a GMS for fungus on all nails we receive.  We often have a difficult
> time keeping the nail tissue on the slide.  We've tried baking for long
> periods, pre-treating in formalin, using silane slides, with no luck. 
> Even
> when the nails cut relatively easily we still lose it, and end up running
> several GMS stains before we might get a speck or two of tissue we can 
> look
> at.  We use the Artisan stainer for our specials.  We are really not
> interested in performing the GMS manually, due to volume and turn around
> time restrictions.  Any suggestions?
>
> Clare J. Thornton, HTL(ASCP)
> Assistant Histology Supervisor
> Dahl-Chase Diagnostic Services
> 417 State Street, Suite 540
> Bangor, ME 04401
> cthornton <@t> dahlchase.com
>
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