[Histonet] Safranin O stain in plastic sections

Peter Carroll carrolpb <@t> umdnj.edu
Fri Jan 30 09:22:28 CST 2009


Gotta second this. I've done a lot of PMMA and/or Spurrs-embedded 
sectioning with it with good results.

Jack Ratliff wrote:
> Yes, it works quite well for both demineralized and undemineralized 
> bone sections embedded in resin. I perform MMA embedding (not a kit) 
> and get really nice undemineralized results. The resin I use allows me 
> the opportunity to deplastify the sections just as one would 
> deparaffinize when working with paraffin.
>
> Jack Ratliff
>
> On Jan 30, 2009, at 8:47 AM, "Markus F. Meyenhofer" 
> <micro <@t> superlink.net> wrote:
>
>> Hello all.
>> Does Safranin O staining work on plastic sections (bone, decalcified)?
>> Any help would be appreciated.
>> Regards,
>> Markus
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