AW: [Histonet] Hi, We have recently started staining liver biopsies routinely with Retic (untoned, no counterstain). We are trying to achieve black reticulin fibres with a clear background. Although we produce clean, crisp Retics we are still left with

[Gudrun Lang]

Do you make the silversolution with ammonia? If the silversolution isn't
properly retitered with silvernitrat, there is an excess amount of ammonia,
that makes a yellowish background.
The silversolution has to be faintly grey.
Gudrun

-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von SARAH
REEVES
Gesendet: Mittwoch, 21. Jänner 2009 18:58
An: histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] Hi, We have recently started staining liver biopsies
routinely with Retic (untoned, no counterstain). We are trying to achieve
black reticulin fibres with a clear background. Although we produce clean,
crisp Retics we are still left with a go

Hi, We have recently started staining liver biopsies routinely with Retic
(untoned, no counterstain).  We are trying to achieve black reticulin fibres
with a clear background.  Although we produce clean, crisp Retics we are
still left with a golden background. We use Sodium Thiosulphate, does anyone
have any other ideas on solutions that would remove this background stain?

Thanks in advance

Sarah Reeves


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