[Histonet] IHC Background

[Igor Deyneko]

Hello everyone!
I was wondering if anyone has ever pre-incubated the antibody with normal
Mouse IgG to get rid of the unspecific staining. The antibody I use
crosreacts with mouse , and both human and mouse tissues stain within a
xenograft. I've heard that one can get rid of the mouse staining by
incubating with murine IgG. Has anyone ever done that and if so, you mind
sharing some info? Thank you in advance.
Igor Deyneko
Infinity Pharmaceuticals
Cambridge, MA