[Histonet] Hi, We have recently started staining liver biopsies routinely with Retic (untoned, no counterstain). We are trying to achieve black reticulin fibres with a clear background. Although we produce clean, crisp Retics we are still left with a golden background. We use Sodium Thiosulphate, does anyone have any other ideas on solutions that would remove this background stain?

[Rene J Buesa]

The black effect is obtained when you tone with gold chloride. The "golden" background will remain, it is "part of the deal".
René J.

--- On Wed, 1/21/09, SARAH REEVES <SARAH.REEVES <@t> ekht.nhs.uk> wrote:

From: SARAH REEVES <SARAH.REEVES <@t> ekht.nhs.uk>
Subject: [Histonet] Hi, We have recently started staining liver biopsies routinely with Retic (untoned, no counterstain). We are trying to achieve black reticulin fibres with a clear background. Although we produce clean, crisp Retics we are still left with a golden background. We use Sodium Thiosulphate, does anyone have any other ideas on solutions that would remove this background stain?
To: histonet <@t> lists.utsouthwestern.edu
Date: Wednesday, January 21, 2009, 12:58 PM

Hi, We have recently started staining liver biopsies routinely with Retic
(untoned, no counterstain).  We are trying to achieve black reticulin fibres
with a clear background.  Although we produce clean, crisp Retics we are still
left with a golden background. We use Sodium Thiosulphate, does anyone have any
other ideas on solutions that would remove this background stain?

Thanks in advance

Sarah Reeves


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